Journal PapersIranian Journal of Horticultural Science , Volume 51 , Issue 1, 2020 May 21, {Pages 139-151 }
Abstract
In this project, the processing ability and quality of green table olives during storage period on six selected cultivars and five genotypes were investigated. After harvesting, debittering carried out using NaOH (2%). The fruits were then placed in a solution of water and salt for fermentation and storage. During the fermentation and storage period, quality parameters such as ash, glucose, fatty acids, acidity, pH, mineral elements and sensory properties wereevaluated. At harvesting time, Direh, Tokhm kabki and KH15 genotypes had the highest fruit weight in comparison to the other selected cultivars and genotypes. During the processing and storage period, there was an increase trend for sodium, while a decrease trend in the amount of calci
Application of polyploidization in breeding of ornamental plants
Maryam Jafarkhani Kermani, Masoumeh Emadpour
Journal PapersFlower and Ornamental Plants , Volume 3 , Issue 2, 2019 March 10, {Pages 77-89 }
Abstract
Plant species with more than two sets of chromosome are called polyploids, which are usually spontaneously created by a mutation during the evolution process. The increased genetic diversity in polyploid plants is considered to be an important strategy in adapting them to new environments which are revolutionizing with time. The changes that are usually associated with auto and allopolyploids include alteration of cell size and morphological or physiological modifications. The aim of this study was to review some aspects of polyploidization and its impact on breeding of ornamental plants. Furthermore, the advantages and disadvantages of the polyploid plants, chromosome doubling agents and application of polyploidization in ornamental plants
Boosting riboswitch efficiency by RNA amplification
Masoumeh Emadpour, Daniel Karcher, Ralph Bock
Journal PapersNucleic acids research , Volume 43 , Issue 10, 2015 March 30, {Pages e66-e66 }
Abstract
Riboswitches are RNA sensors that regulate gene expression in response to binding of small molecules. Although they conceptually represent simple on/off switches and, therefore, hold great promise for biotechnology and future synthetic biology applications, the induction of gene expression by natural riboswitches after ligand addition or removal is often only moderate and, consequently, the achievable expression levels are not very high. Here, we have designed an RNA amplification-based system that strongly improves the efficiency of riboswitches. We have successfully implemented the method in a biological system for which currently no efficient endogenous tools for inducible (trans)gene expression are available: the chloroplasts o
Journal PapersInternational Journal of Agricultural Technology , Volume 11 , Issue 5, 2015 January , {Pages 1263-1273 }
Abstract
This research was conducted to study the effect of removal of the ethylene using potassium permanganate coated nano zeolites from the storage on the quality characteristics and shelf life of peach and nectarine. Potassium permanganate and Zeolite based Nano-Molecular Filters were used in ETH 1500 machine. Two cultivars of peach and three cultivars of nectarine were used in this experiment Commercial traits affecting the marketing and shelf-life of peach and nectarine ie pH, texture (firmness), appearance, total soluble solid concentration (TSS), titratable acidity, internal breakdown and the reduction in fresh weight were measured at the beginning of the experiment (day 0), 12, 24 and 36 days after storage. The experiment was laid in a two
Effect of potassium permanganate-coated nano-zeolites on cherry quality and shelf life.
M Emadpour, B Ghareyazie, Y Rezaei Kalaj, A Omrani, GH Mohammadi
Journal PapersJournal of Agricultural Engineering Research (Iran) , Volume 10 , Issue 1, 2009 January , {Pages 26-Nov }
Abstract
see more details can be augmented. The removal of ethylene can delay over-ripening, prevent spoilage and increase the shelf-life of harvested fruit. This study investigated the effect of removing ethylene from storage using nano particles. Potassium permanganate potassium permanganate Subject Category: Chemicals and Chemical Groupssee more details and two types of zeolite-based nano-molecular filters were used. Small 5 g sachets were placed directly onto the fruit in each container or an ETH 1500 machine (BIOCONSERVACION) was used. The experimental design experimental design Subject Category: Techniques, Methodologies and Equipment
Effect of ethylene absorption using nano-particles on the storage and quality characteristics of apricot
M Emadpour, Middle East Bioresearchers, Iran Teran, B Ghareyazie, Gh Mohammadi
Journal PapersIn Agronomy and Horticulture , 2009 January , {Pages }
Abstract
Internal and external accumulation of ethylene is considered as one of the principle causes of post-harvest losses in several fruits including apricots. Ethylene is an important factor in the ripening of fruits and its production and accumulation during the storage can cause serious damage to the stored apricot. Furthermore, spoilage due to indigenous bacteria in presense of ethylene can also be augmented. Reduced quality characters of the fruit, results in the reduced export of apricots and causes seasonal fluctuation of the price and availability. Ethylen can be removed from the environment in the storage in different ways. This study was conducted to evaluate the effects of ethylene absorption through nanoparticles coated by potassium pe
In temperate regions, subsequent to apple, pear is one of the important crops. Traditional pear breeding involves hybridization between and within different species. However, due to high levels of heterozygosity, polygenic characters and long juvenile stage, traditional breeding is considered to be very time consuming and expensive. Therefore, using modern biotechnology methods such as genetic engineering has become popular. In the present investigation gene transformation of two pear cultivars (" Harrow Delight" and" Bartlett") using agrobacterium were studied. In gene transformation by agrobacterium, EHA101 and LBA4404 strains of agrobacterium harboring pBI121-GUS and kanamycin resistant nptII gene were used. The following parameters: typ
Genetic engineering technique could be used to increase rape seed yield and also increase the disease and pest resistance in this valuable crop. Genetic engineering to microspores and production of haploid cultures is one of the new techniques which is being used recently. The main technique for haploid production in Brasica is microspore culture, which the technique has been registered for many species and important commercial genotypes. The first stage in gene transformation to microspore is identification of the developmental stages of the microspore growth and also the factors affecting its isolation and embryogenesis. In the present investigation the factors affecting gene transformation of rape seed microspores using the gus gene were
Effect of the removal of ethylen hormone by potassium permangenate coated zeolite nanoparticles on the increased quality and quantity of storage of iceberg lettuce (lactuca?…
KALAJ Y REZAEI, BEHZAD GHARAHYAZI, M EMADPOUR, A OMRANI
Internal and external accumulation of ethylene is considered as one of the most significant causes of post-harvest losses in cut leafy vegetables including lettuce and cabbage. Ethylene is an important factor in the ripening of fruits and is naturally produced in most plant tissues. Its production and accumulation during the storage can cause serious damage to the stored vegetables such as lettuce and cabbage. It plays a significant role in shelf life and can cause a marked increase in respiration rates and enhanced senescence. In vegetables and cut flowers, accelerated aging and the initiation of ripening can occur following exposure to ethylene at concentrations as low as 0.1 ml/l. As senescence begins, spoilage due to indigenous bacteria
Preparation of suitable construct and optimization of direct and indirect regeneration of roses for gene transformation
Ali-Akbar Habashi
Journal Papers , 2008 January , {Pages }
Abstract
Powdery mildew disease causes yield and quality reduction in plants of Rosaceae family every year. In order to create resistant rose plants to this disease, plant regeneration was first optimized. The best regeneration was obtained using leaf explants cultured in medium containing 2, 4-D and NAA. After optimizing tissue culture stage, changing the construct containing Kitinase gene (chi) was done by selecting suitable vector and its transfer to E. coli cells and to Agrobacterium tumofacience. This was achieved by cloning Kitinase protein encoding gene of one kb length into pGEM-7Zf (+) transporter. This plasmid was transferred to isolate XLI-Blue of E. coli. Conjugated Kitinase gene was subsequently cut using two restriction enzymes and the
Traditional pear breeding involves hybridization between and within different species. However, due to high levels of heterozygosity, polygenic characters and long juvenile stage, traditional breeding is very time consuming and expensive. Therefore, using modern biotechnology methods such as genetic engineering and mutation has become popular. In this regard effective and repeatable in vitro regeneration protocols are the prerequisites for in vitro breeding. The aim of the present project was to develop an effective direct and indirect regeneration method for three commercially important pear cultivars (Dare Gazy, Harrow Delight and Bartlett). For direct regeneration; culture media (Nitsch) containing different concentrations of TDZ (0, 2.5
This research was carried out in Gene Transfer and Tissue Culture Laboratory of Agricultural Biotechnology Research Institute of Iran (ABRII) in 2008. Fire blight caused by the bacterium, Erwinia amylovra (Burrill), reduces the yield and quality of Pomoideae growing areas annually. The aim of this study was isolation and cloning of the gene encoding harpin protein (hrpN) from E. amylovra strain 273 and construction of pBI-hrpN recombinant plasmid in order to widespread fire blight resistance strategy development based on expression of defense response of pear (Pyrus communis). Specific primers (forward and reverse) were designed for amplifying the gene (hrpN) using complete sequence of genomic DNA of E. amylovora strain 273. Then, to carry
Effect of the removal of ethylene hormone by potassium permanganate coated zeolite nanoparticles on the increased quality and quantity of storage of iceberg lettuce (Lactuca?…
Y Kalaj, B Ghareyazie, M Emadpour, A Omrani
Journal PapersJournal of Agricultural Sciences and Natural Resources , Volume 15 , Issue 6, 2008 January , {Pages 188-197 }
Effect of the removal of ethylene hormone by potassium permanganate coated zeolite nanoparticles on the increased quality and quantity of storage of iceberg lettuce (Lactuca …
Effect of the Removal of Ethylen Hormone by Potassium Permangenate Coated Zeolite Nanoparticles on the Increased Quality and Quantity of Storage of Iceberg Lettuce (Lactuca …